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Updated: Aug 22, 2026

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
[Inhibition of hepatitis B virus replication by RNA interference in vitro]
Cai Zhu1, Xue-Gong Fan, Ning Li
1Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To design pSilencer3.1-H1hygro plasmid expressing short interfering RNAs (siRNA) that targets HBV core gene region, and to evaluate inhibitory effect of this siRNA on HBV in vitro.
Methods:
HepG2 2.2.15 was used as target cells. The plasmid and liposome metafectene were cotransfected into the cultured cells, HBV DNA were analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR.
Results:
The plasmid expressing siRNA was successfully constructed. The two constructed siRNAs could effectively inhibit HBV replication, and their inhibitive effect on HBV was dose-dependent.
Conclusion:
These results showed that siRNA could substantially inhibit HBV replication in the infected cells
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