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Updated: Aug 22, 2026

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
Published on: April 13, 2015
[The anti-cancer effect of siRNA targeting human telomerase reverse transcriptase in SMMC-7721 cells]
Su-Jun Zheng1, Yun Xia, Hong Ren
1Institute for Viral Hepatitis and the Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China.
Objective:
To explore the anti-cancer effects of siRNAs targeting hTERT in SMMC-7721 cells.
Methods:
Two siRNAs targeting hTERT mRNA were designed and synthesized by T7 transcription system in vitro. MMT, RT-PCR and Western blot were applied to evaluate effects on inhibiting cell growth, hTERT mRNA and protein expression in SMMC-7721 cells.
Results:
siRNAs decreased cell proliferation in a dose-dependent manner. At a concentration of 100 nmol/L, siRNAs exhibited obvious effects on inhibiting hTERT mRNA and protein expression in SMMC-7721 cells.
Conclusion:
siRNAs targeting hTERT have significant inhibitory effects on hTERT gene expression in SMMC-7721 cells. siRNA has the possibility to become a new anti-cancer agent
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