Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Decellularized human tissue engineering aortic valves conduit].

Bin Cui1, Ying-long Liu, Ning Xie

  • 1Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China.

Zhonghua Yi Xue Za Zhi
|September 25, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical coherence tomography-derived macrophage arc as a novel biomarker for predicting adverse cardiovascular events in coronary artery disease: a multicentre study.

European heart journal. Imaging methods and practice·2026
Same author

Low-substrate nitrogen drives functional succession toward a cooperative Candidatus Brocadia consortium in anammox systems.

Bioresource technology·2026
Same author

SAS-bench: A fine-grained benchmark for evaluating short answer scoring with large language models.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Integrated metagenomics unravels the microbial mechanisms driving greenhouse gas and odor emissions during composting.

Bioresource technology·2026
Same author

Phytohormone-based metabolic regulation: From endogenous secretion discovery to Indole-3-butyric acid strategies for enhancing low-temperature denitrification.

Bioresource technology·2026
Same author

Work function-regulated two-dimensional porous C<sub>7</sub>N<sub>6</sub>-based single-atom catalysts for the hydrogen evolution reaction.

Physical chemistry chemical physics : PCCP·2026
Same journal

[Systematic review and meta-analysis of the prevalence of small intestinal bacterial overgrowth in patients with functional gastrointestinal disorders].

Zhonghua yi xue za zhi·2026
Same journal

[Dendritic cell and/or PD-1 monoclonal antibody therapy induces intratumoral remodeling of CD8⁺T cells in humanized cerebral glioblastoma mice models].

Zhonghua yi xue za zhi·2026
Same journal

[Study on expression characteristics of complement C3 in childhood asthma and its mechanism regulating macrophage migration and polarization involved in immune inflammatory response].

Zhonghua yi xue za zhi·2026
Same journal

[Consistency evaluation of the domestic Lifotronic platform and foreign luminescence platforms in detecting core cerebrospinal fluid biomarkers in Chinese Alzheimer disease patients].

Zhonghua yi xue za zhi·2026
Same journal

[Analysis of clinical characteristics and risk factors in children and adolescents with narcolepsy type 1 across different severities of daytime sleepiness].

Zhonghua yi xue za zhi·2026
Same journal

[Standard operating procedure for pre-analytical blood biomarker processing in Alzheimer's disease (2026 edition)].

Zhonghua yi xue za zhi·2026
See all related articles

A new decellularization method using 1% deoxycholate (DOA) and DNase effectively removes cellular material from human homograft aortic valves. This process preserves the valve

Area of Science:

  • Tissue Engineering
  • Biomaterials Science
  • Immunology

Context:

  • Human homograft aortic valves are crucial for cardiovascular repair but face immunogenicity challenges.
  • Developing effective decellularization techniques is vital for creating safe and functional tissue-engineered grafts.
  • Current methods may compromise structural integrity or fail to adequately remove immunogenic components.

Purpose:

  • To establish an optimal decellularization protocol for human homograft aortic valves.
  • To evaluate the biological, immunological, and biomechanical properties of decellularized aortic valve conduits.
  • To assess the potential of these conduits for future tissue engineering applications.

Summary:

  • A novel decellularization method utilizing isotonic buffer with 1% deoxycholate (DOA) and DNase/RNase was developed for human homograft aortic valves.

Related Experiment Videos

  • This method achieved complete decellularization while preserving the extracellular matrix structure, significantly reducing DNA and HLA-DR antigen content.
  • Biomechanical properties remained largely unchanged, with increased water content in the decellularized aortic wall.

    • Impact:

    • The optimized decellularization protocol yields a homograft aortic valve conduit with reduced immunogenicity and preserved structural and mechanical integrity.
    • This decellularized valve serves as a promising candidate for direct clinical use or as a scaffold for host recellularization in tissue-engineered valve development.
    • This research advances the field of regenerative medicine by providing a viable option for aortic valve replacement and repair.