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Updated: Aug 22, 2026

Elucidating β-1,3-Glucanase and Peroxidase Physicochemical Properties of Wheat Cell Wall Defense Mechanism Against Diuraphis noxia Infestation
Published on: July 26, 2024
Wheat pathogenesis-related proteins of class 4 have ribonuclease activity
Carlo Caporale1, Iris Di Berardino, Luca Leonardi
1Dipartimento di Agrobiologia ed Agrochimica, Università della Tuscia, via S. Camillo de Lellis, 01100 Viterbo, Italy.
Abstract:
We have demonstrated that wheatwin1, a wheat pathogenesis-related protein of class 4 (PR4), has ribonuclease activity. Both native and recombinant proteins hydrolyse RNA from wheat coleoptils and have antifungal activity. Sepharose-bound wheatwin1 is able to interact with either wheat or Fusarium culmorum RNA. 3D modelling studies showed that, like ribonucleases A and T1, the action mechanism should involve two His residues, an Arg residue and an Asp residue.
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