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Pseudomonas psychrotolerans sp. nov
Elke Hauser1, Peter Kämpfer2, Hans-Jürgen Busse3,1
1Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Vienna, Austria.
International Journal of Systematic and Evolutionary Microbiology
|September 25, 2004
Summary
A novel bacterial species, Pseudomonas psychrotolerans, was identified from veterinary samples. This yellow-pigmented bacterium is distinct from its closest relative, Pseudomonas oleovorans, based on genetic and biochemical analyses.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Bacterial isolates were obtained from veterinary clinical samples.
- Initial analysis suggested classification within the genus Pseudomonas.
Purpose of the Study:
- To characterize and classify novel bacterial strains isolated from veterinary samples.
- To determine the phylogenetic relationship of strain C36T to known Pseudomonas species.
Main Methods:
- 16S rRNA gene sequencing for phylogenetic analysis.
- Chemotaxonomic analyses including ubiquinone, polyamine, and fatty acid profiling.
- Genomic fingerprinting and DNA-DNA hybridization for species delineation.
Main Results:
- Strain C36T showed 99.5% 16S rRNA gene similarity to Pseudomonas oleovorans.
- Chemotaxonomic and genomic data differentiated strain C36T from P. oleovorans.
- DNA-DNA hybridization confirmed strain C36T as a distinct species.
Conclusions:
- Strain C36T represents a novel species within the genus Pseudomonas.
- The proposed name for this new species is Pseudomonas psychrotolerans.
- Further investigation suggests P. oleovorans ATCC 29347 may not belong to P. oleovorans sensu stricto.