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Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
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Published on: January 3, 2015

A deletion affecting several gene candidates is present in the Evergrowing peach mutant.

D G Bielenberg1, Y Wang, S Fan

  • 1Department of Horticulture, Clemson University, Clemson, SC 29634, USA. dbielen@clemson.edu

The Journal of Heredity
|September 25, 2004
PubMed
Summary

The Evergrowing (EVG) peach mutation, affecting winter dormancy, is caused by a large deletion. This deletion impacts multiple MADS-box genes, disrupting normal bud set and growth cessation in perennial species.

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Area of Science:

  • * Plant genetics
  • * Molecular biology
  • * Woody perennial physiology

Background:

  • * The Evergrowing (EVG) peach mutant exhibits a failure to enter winter dormancy, characterized by continuous growth and lack of terminal bud formation.
  • * This unique trait in woody perennials is controlled by a single recessive nuclear gene.
  • * Previous research established a genetic linkage map and a bacterial artificial chromosome (BAC) contig containing the EVG mutation.

Purpose of the Study:

  • * To identify the genetic basis of the EVG mutation in peach.
  • * To characterize the molecular nature of the mutation affecting winter dormancy.
  • * To investigate the role of MADS-box genes in regulating dormancy in perennial plants.

Main Methods:

  • * Development of a molecular genetic linkage map using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers.
  • * Assembly of a bacterial artificial chromosome (BAC) contig encompassing the EVG locus.
  • * Polymorphism detection using a MADS-box gene probe and RFLP analysis to bracket the deletion.

Main Results:

  • * A significant deletion was identified in the EVG peach genome, confirmed by probe hybridization and RFLP analysis.
  • * The deletion spans a region of approximately 180 kb and affects at least four of six potential MADS-box transcription factor sequences.
  • * This deletion is the likely cause of the altered dormancy phenotype in EVG peach.

Conclusions:

  • * The EVG mutation in peach is caused by a substantial genomic deletion impacting multiple MADS-box transcription factors.
  • * These MADS-box genes play a critical role in regulating essential developmental processes like winter dormancy in woody perennials.
  • * Understanding this mutation provides insights into the genetic control of dormancy and perennial plant development.