Related Experiment Video
Updated: Aug 22, 2026
09:55
From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Expression, crystallization and preliminary crystallographic analysis of YciE, a stress protein from Escherichia coli
Deqian Liu1, Yonghong Zhao, Xiuzhen Fan
1The Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, TX 77555-0647, USA.
Acta Crystallographica. Section D, Biological Crystallography
|September 25, 2004
Abstract:
The stress protein Escherichia coli YciE was overexpressed and purified in three chromatographic steps. Crystals were obtained using PEG 4000 as a precipitant. The YciE protein crystals diffracted to 3.0 A resolution using a rotating-anode X-ray source. The lattice type is rhombohedral, with unit-cell parameters a = b = 64.2, c = 167.9 A, alpha = beta = 90, gamma = 120 degrees. The crystal belongs to space group R32.
