Related Experiment Video
Updated: Aug 22, 2026

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
Published on: July 17, 2018
Towards the automatic analysis of 1H NMR spectra: Part 4--additional requirements of flow-NMR
1AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire, SK10 4TG, UK. lee.griffiths@astrazeneca.com
Abstract:
Flow-NMR allows more rapid and convenient acquisition of NMR spectra. Its main application area has therefore been in multiple parallel synthesis or combinatorial chemistry. At the same time, there is a significant need to automate the analysis of the resultant spectra. However, flow-NMR brings spectral imperfections, which compromise attempts to automate this analysis. This study proposes experimental and computational expedients to accommodate the effects of residual solvent peaks, 13C satellites, finite signal-to-noise ratio, impurities, presaturation on integral calculations, the 'silent' region and how multiplet areas can be scaled to numbers of protons in this environment.
Related Concept Videos
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
¹H NMR Signal Integration: Overview
NMR Spectrometers: Overview
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
Two-Dimensional (2D) NMR: Overview
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
2D NMR: Overview of Heteronuclear Correlation Techniques

