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Cell-dependent posttranslational processing and secretion of recombinant mouse renin-2
M Paul1, N Nakamura, R E Pratt
1Division of Cardiovascular Medicine, Falk Cardiovascular Research Center, Stanford University, California 94305.
The American Journal of Physiology
|February 1, 1992
Summary
Mouse renin (Ren-2d) processing and secretion differ significantly between cell types. Specific cellular factors, like processing enzymes, influence how prorenin is converted to active renin.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Biology
Background:
- Renin, primarily expressed from the Ren-2d gene in DBA/2 mouse submandibular glands (SMG), undergoes complex processing.
- Prorenin is synthesized and converted to a single-chain intermediate, then to a mature two-chain (2C) form for regulated secretion.
Purpose of the Study:
- To investigate if renin processing is dependent on the specific cell type.
- To compare renin processing and secretion in transfected Chinese hamster ovary (CHO) cells and mouse pituitary AtT-20 cells with native SMG.
Main Methods:
- Stable integration of renin (Ren-2d) complementary DNA into CHO and AtT-20 cell genomes via transfection.
- Examination of renin processing and secretion patterns in these transfected cell lines.
Main Results:
- Transfected CHO cells secreted only prorenin.
- Transfected AtT-20 cells secreted both prorenin and active renin, processing prorenin to a single-chain (1C-renin) form, but not to the mature 2C form.
- Prorenin to 1C-renin conversion was slower in AtT-20 cells compared to SMG.
Conclusions:
- Renin biosynthesis and secretion exhibit significant cell-dependent variations.
- Cellular characteristics, including the presence of specific processing enzymes, play a crucial role in determining mouse renin processing pathways.