Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Can peritoneal B cells be rendered unresponsive?

L B Liou1, G L Warner, D W Scott

  • 1Immunology and Immunotherapy Division, University of Rochester Cancer Center, NY.

International Immunology
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic advances and gradual improvement in overall survival in mantle cell lymphoma over four decades.

British journal of haematology·2025
Same author

Long-term follow-up of bulky classic Hodgkin lymphoma managed with ABVD and PET-guided RT demonstrates excellent outcomes in PET-negative cases.

British journal of haematology·2024
Same author

Transverse Fracture of the Axis.

The Journal of comparative medicine and veterinary archives·2022
Same author

Pleuro-Pneumonia, with Subsequent Scorbutus.

Atlanta medical and surgical journal·2022
Same author

Letter to the Editor.

Veterinary dermatology·2021
Same author

10<sup>th</sup> European immunogenicity platform open symposium on immunogenicity of biopharmaceuticals.

mAbs·2020
Same journal

Thymic cortical epithelial Psmb11-encoded β5t in mouse and human.

International immunology·2026
Same journal

Signaling and Transcriptional Control of Thymocyte Differentiation.

International immunology·2026
Same journal

17,18-epoxyeicosatetraenoic acid and its metabolite attenuate IL-33-induced airway inflammation involving group 2 innate lymphoid cells.

International immunology·2026
Same journal

FcμR enhances recall responses by promoting the generation of CD80+PD-L2+ memory B cells.

International immunology·2026
Same journal

Mitochondrial Transfer-Driven Immune Evasion in the Tumor Microenvironment.

International immunology·2026
Same journal

Granulocyte heterogeneity in immune-mediated and inflammatory diseases: Insights from single-cell transcriptomic analyses.

International immunology·2026
See all related articles

Peritoneal B cells (PBCs) are resistant to tolerance induction, unlike splenic B cells. This resistance may stem from defective signaling pathways, suggesting PBCs have unique regulatory mechanisms in the immune system.

Area of Science:

  • Immunology
  • B cell biology

Background:

  • Ly-1+ B cells, abundant in mouse peritoneum, produce autoantibodies and influence B cell repertoire.
  • The regulatory capacity of these peritoneal B cells (PBCs) remains largely unknown.

Purpose of the Study:

  • To investigate the sensitivity of PBCs compared to splenic B cells to tolerance induction.
  • To explore potential defects in PBC signaling pathways.

Main Methods:

  • Culturing normal splenic or peritoneal B cells with anti-immunoglobulin (Ig) reagents.
  • Challenging cells with haptens (fluorescein, trimethylammonium) or lipopolysaccharide (LPS).
  • Measuring IgM responses and analyzing calcium (Ca2+) signaling.

Main Results:

  • PBCs demonstrated significant resistance to tolerance induction, unlike splenic B cells which showed up to 90% reduced responsiveness.

Related Experiment Videos

  • The anti-trimethylammonium response in PBCs, linked to VH11 usage, was also resistant to tolerance.
  • PBCs exhibited a blunted initial Ca2+ response to anti-Ig, suggesting defective signaling.

    • Conclusions:

    • Peritoneal B cells are largely resistant to conventional tolerance induction mechanisms.
    • Defective signaling pathways, potentially involving protein kinase C, may underlie PBC resistance.
    • PBCs may possess distinct regulatory properties compared to splenic B cells.