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Identification and characterization of an Onchocerca volvulus cDNA clone encoding a microfilarial surface-associated
S Lustigman1, B Brotman, E H Johnson
1Laboratory of Virology and Parasitology, Lindsley F. Kimball Research Institute of the New York Blood Center, NY 10021.
Molecular and Biochemical Parasitology
|January 1, 1992
Summary
Researchers identified a new antigen (OV103) from Onchocerca volvulus, the parasite causing river blindness. This surface antigen shows promise for diagnosing and potentially treating onchocerciasis, especially in early infection stages.
Area of Science:
- Parasitology
- Molecular Biology
- Immunology
Background:
- Onchocerca volvulus is a parasitic nematode responsible for onchocerciasis (river blindness).
- Identifying parasite antigens is crucial for developing diagnostic tools and therapeutic strategies.
Purpose of the Study:
- To identify and characterize a recombinant cDNA clone expressing a microfilarial surface antigen of Onchocerca volvulus.
- To evaluate the diagnostic potential and functional activity of the identified antigen.
Main Methods:
- Screening of a lambda gt11 cDNA expression library using chimpanzee antiserum.
- Characterization of the cDNA clone (OV103) and the encoded protein.
- Serological analysis of patient sera and in vitro killing assays.
Main Results:
- A cDNA clone, OV103, encoding a 12.5-kDa protein was isolated, corresponding to a 15-kDa parasite antigen.
- The antigen is located on the surface of microfilariae and in the cuticle/hypodermis of adult worms.
- OV103 was recognized by 89% of sera from individuals with low patent infection levels.
- Antibody response to OV103 preceded microfilariae appearance in low-infection chimpanzees.
- Antibodies against OV103 mediated in vitro killing of microfilariae.
Conclusions:
- OV103 represents a promising surface-associated antigen of Onchocerca volvulus.
- The antigen shows potential for early diagnosis of onchocerciasis and as a target for immunotherapies.