Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The synapsin I brain distribution in ischemia.

K Kitagawa1, M Matsumoto, K Sobue

  • 1First Department of Internal Medicine, Osaka University, Japan.

Neuroscience
|January 1, 1992
PubMed
Summary

Synapsin I, a marker for synaptic vesicles, effectively highlights ischemic damage in gerbil brains. Its distribution reveals presynaptic terminal injury and recovery following cerebral ischemia.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of manidipine hydrochloride on the renal microcirculation in spontaneously hypertensive rats.

Journal of cardiovascular pharmacology·1992
Same author

[Prediction formulas for pulmonary function tests expressed in linear and exponential form for healthy Japanese adults].

Nihon Kyobu Shikkan Gakkai zasshi·1992
Same author

[Genetic analysis of the toxins produced by Clostridium botulinum].

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·1992
Same author

[The introduction of home mechanical ventilation in Japan].

Nihon Kyobu Shikkan Gakkai zasshi·1992
Same author

Helical volume CT and its clinical significance.

Fukushima journal of medical science·1992
Same author

Inactivation of Bacillus subtilis glutamine synthetase by metal-catalyzed oxidation.

Journal of biochemistry·1992

Area of Science:

  • Neuroscience
  • Cerebrovascular Research
  • Immunohistochemistry

Background:

  • Synapsin I is a key protein localized in synaptic vesicles within the brain's neuropil.
  • Understanding its distribution aids in assessing neuronal damage, particularly in presynaptic terminals.
  • Cerebral ischemia models are crucial for studying neuronal injury and potential recovery mechanisms.

Purpose of the Study:

  • To map the distribution of synapsin I in the normal gerbil brain.
  • To investigate the utility of synapsin I as a marker for ischemic damage to presynaptic terminals.
  • To compare the temporal profile of synapsin I changes with other markers like microtubule-associated protein 2.

Main Methods:

  • Immunohistochemistry was employed to visualize synapsin I distribution in gerbil brain tissue.

Related Experiment Videos

  • Gerbil models of unilateral and bilateral cerebral ischemia were utilized.
  • Immunoblot analysis confirmed protein breakdown in affected areas.

    • Main Results:

    • Synapsin I immunoreactivity was primarily observed in the neuropil of normal gerbil brains.
    • In ischemic hemispheres, synapsin I loss correlated with infarction areas and occurred later than MAP2 loss.
    • Wallerian degeneration and subsequent resynaptogenesis were detectable via synapsin I changes in the dentate gyrus molecular layer.

    Conclusions:

    • Synapsin I is a reliable marker for presynaptic terminal integrity and damage in cerebral ischemia.
    • Its distinct temporal changes aid in differentiating acute injury from later degenerative processes.
    • Synapsin I's persistent presence in CA1 hippocampus post-ischemia may indicate complex injury patterns.