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Related Experiment Videos

Maturational changes in airway smooth muscle structure-function relationships.

H B Panitch1, K S Deoras, M R Wolfson

  • 1Department of Pediatrics, St. Christopher's Hospital for Children, Temple University School of Medicine, Philadelphia, Pennsylvania 19134-1095.

Pediatric Research
|February 1, 1992
PubMed
Summary

Airway smooth muscle (ASM) in sheep becomes stronger and more sensitive to acetylcholine with age. These changes in ASM contractility occur throughout maturation, impacting airway function.

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Area of Science:

  • Pulmonary Physiology
  • Developmental Biology
  • Smooth Muscle Biology

Background:

  • Airway compliance decreases with age.
  • Airway smooth muscle (ASM) exhibits parallel changes to the trachea during development.
  • Both passive and active stress in ASM increase from preterm to adult stages.

Purpose of the Study:

  • To investigate the maturational changes in sheep airway smooth muscle (ASM).
  • To quantify the alterations in passive and active stress, contractility, and sensitivity to agonists during ASM development.

Main Methods:

  • Trachealis muscles from sheep across five age groups (gestation to adult) were isolated.
  • Passive and active stress were measured at optimal length.
  • Concentration-effect curves for acetylcholine (ACh) and KCl were generated.

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  • Morphometric analysis was performed using computerized image analysis.

    • Main Results:

    • Passive and active stress in ASM significantly increased with maturation (p < 0.001).
    • Active stress in response to ACh and KCl increased with dose and age (p < 0.002).
    • The ED50 for ACh decreased with maturation (p < 0.005), indicating increased sensitivity.
    • Muscle dimensions increased, but the contractile to connective tissue ratio remained constant.

    Conclusions:

    • Airway smooth muscle (ASM) demonstrates progressive increases in contractility and acetylcholine sensitivity during maturation.
    • These maturational changes in ASM contribute to age-related alterations in airway mechanics.
    • Understanding these developmental changes is crucial for respiratory physiology research.