Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vitamin D-mediated gene expression.

K E Lowe1, A C Maiyar, A W Norman

  • 1Department of Biochemistry, University of California, Riverside 92521.

Critical Reviews in Eukaryotic Gene Expression
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of the second messengers involved in 1α,25-dihydroxyvitamin D(3) stimulated intestinal calcium absorption (transcaltachia).

Endocrine·2010
Same author

Preferential accumulation in vivo of 24R,25-dihydroxyvitamin D(3) in growth plate cartilage of rats.

Endocrine·2010
Same author

Photoprotection by 1alpha,25-dihydroxyvitamin D and analogs: further studies on mechanisms and implications for UV-damage.

The Journal of steroid biochemistry and molecular biology·2010
Same author

In vivo relevance for photoprotection by the vitamin D rapid response pathway.

The Journal of steroid biochemistry and molecular biology·2007
Same author

Skin cancer prevention: a possible role of 1,25dihydroxyvitamin D3 and its analogs.

The Journal of steroid biochemistry and molecular biology·2005
Same author

1,25-Dihydroxyvitamin D and three low-calcemic analogs decrease UV-induced DNA damage via the rapid response pathway.

The Journal of steroid biochemistry and molecular biology·2004

The steroid hormone 1,25(OH)2D3, or calcitriol, regulates gene expression through the vitamin D receptor (VDR). This interaction influences cell growth, differentiation, and mineral balance, highlighting calcitriol's crucial role in biological processes.

Area of Science:

  • Molecular Endocrinology
  • Gene Regulation
  • Steroid Hormone Action

Background:

  • The steroid hormone 1,25(OH)2D3 (calcitriol) is a key regulator of gene expression.
  • Calcitriol influences cellular processes like proliferation, differentiation, and mineral homeostasis.
  • Its effects are mediated through the vitamin D receptor (VDR), a nuclear receptor.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which 1,25(OH)2D3 modulates gene expression.
  • To understand the role of the vitamin D receptor (VDR) in mediating these genomic effects.
  • To explore the critical DNA sequences and factors involved in vitamin D-dependent gene regulation.

Main Methods:

  • Review of experimental techniques used to study 1,25(OH)2D3 and VDR interactions.

Related Experiment Videos

  • Analysis of VDR's function as a transcription factor binding to hormone response elements (HREs).
  • Examination of the structural requirements of HREs for specific receptor binding.

    • Main Results:

    • 1,25(OH)2D3 modulates gene expression in a tissue- and developmental-specific manner.
    • The VDR, upon ligand binding, acts as a transcription factor targeting specific DNA sequences (HREs).
    • HRE sequence, spacing, and orientation are critical for VDR binding and gene regulation, with influence from co-factors like fos and jun.

    Conclusions:

    • The vitamin D receptor (VDR) is central to the genomic actions of 1,25(OH)2D3.
    • Precise DNA binding by VDR to HREs is essential for regulating target gene expression.
    • Understanding these mechanisms provides insight into the broad biological impact of vitamin D.