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Updated: Aug 14, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Minor-groove width and accessibility in B-DNA drug and protein complexes
1Cancer Research Campaign Biomolecular Structure Unit, Institute of Cancer Research, Sutton, Surrey, UK.
Abstract:
A new definition is presented for minor-groove width in double-helical B-DNA structures. This uses interstrand H4' ... H5' rather than P ... P distances. It is shown by examination of various oligonucleotide crystal structures that these H4' ... H5' distances are a sensitive measure of minor-groove drug and protein binding, since these hydrogen atoms are in direct non-bonded contact with such bound ligands.
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