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Construction and characterization of a fusion protein with epidermal growth factor and the cell-binding domain of
Y Kawase1, Y Ohdate, T Shimojo
1Biotechnology Research Laboratories, Takara Shuzo Co. Ltd., Shiga, Japan.
FEBS Letters
|February 24, 1992
Abstract:
An efficient expression system was constructed for C-EGF, a fusion protein made of a fragment of the cell-binding domain of human fibronectin (FN) bound with epidermal growth factor (EGF). C-EGF was produced in Escherichia coli HB101 cells carrying the recombinant plasmid pCE102 as inclusion bodies, which were solubilized and refolded after purification. C-EGF had both cell-adhesive and EGF activities, so it might be more effective than EGF in therapeutic applications. This fusion system would be useful for the construction of a recombinant drug delivery system for cells that have fibronectin receptors (integrins).