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Construction of a broad-host-range non-mobilizable stable vector carrying RP4 par-region
J Crouzet1, S Lévy-Schil, L Cauchois
1Département Biotechnologies, Rhône-Poulenc Rorer S.A., Vitry sur Seine, France.
Gene
|January 2, 1992
Abstract:
Plasmid pXL1635 was constructed from the already segregationally stable incP-derived pRK290. Plasmid pXL1635 should be suitable for industrial and environmental uses in Gram- bacteria since (i) it contains the par fragment from RP4 which increases its stability in Pseudomonas denitrificans, a cobalamin-producing and industrially used bacterium, and (ii) the RK2 oriT has been deleted, leading to a non-mobilizable plasmid.