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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The phylogenetic position of Spathulospora based on DNA sequences from dried herbarium material
Patrik Inderbitzin1, Sea Ra Lim, Brigitte Volkmann-Kohlmeyer
1Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada. bhpatrik@mail.botany.ubc.ca
Abstract:
The phylogenetic position of the marine ascomycete genus Spathulospora was investigated using partial SSU and LSU DNA sequences obtained from dried herbarium specimens. Spathulospora was represented by the two species S. adelpha and S. antarctica. Phylogenetic analyses using Bayesian, parsimony, and neighbour-joining algorithms on SSU and LSU data sets agreed with the placement of Spathulospora. Both Spathulospora species are each others closest relatives, and group within the Lulworthiales (Sordariomycetes, Ascomycota) with support in all analyses. Members of the morphologically similar insect parasites in the Laboulbeniomycetes are not closely related to Spathulospora. Despite several striking morphological differences between Spathulospora and Lulworthiales, an important shared morphological character was found that until now had not been recognized. Ascospores of Spathulospora and some members of the Lulworthiales have apical chambers containing mucus believed to be involved in ascospore attachment. A closest relative to Spathulospora could not be determined.
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