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Published on: October 25, 2017
Hydrodynamics of nanoscopic tubulin rings in dilute solutions
Hacène Boukari1, Ralph Nossal, Dan L Sackett
1Laboratory of Integrative and Medical Biophysics, NICHD, Bethesda, Maryland 20892, USA. boukarih@mail.nih.gov
Abstract:
We combine fluorescence correlation spectroscopy and sedimentation velocity measurements to probe the hydrodynamic behavior of tubulin dimers and nanoscopic tubulin rings. The rings are rigid, have circular geometry, and are monodisperse in size. We use the high-precision ratio of the sedimentation coefficients and that of the translational diffusion coefficients to validate theories for calculating the hydrodynamic properties of supramolecular structures.
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