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Updated: Aug 21, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Elastic properties of a single-stranded charged homopolymeric ribonucleotide
Yeonee Seol1, Gary M Skinner, Koen Visscher
1Department of Physics, University of Arizona, 1118 E. 4th Street, Tucson, Arizona 85721, USA.
Abstract:
We have investigated the elastic properties of poly(U), homopolymeric single-stranded RNA molecules that lack any base pairing and stacking interactions and conform to a random-coil structure. Using single-molecule stretching experiments we show that the elastic properties are described by a wormlike chain model for polymer elasticity rather than by a freely jointed chain model as is commonly used for single-stranded DNA. At low [Na+], introduction of a scale-dependent persistence length is required to account for electrostatic contributions.
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