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Updated: Aug 9, 2026

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
Published on: March 3, 2016
[Hominis placenta promotes melanocyte proliferation melanin synthesis and tyrosinase activity in vitro]
1Department of Traditional Chinese Medicine, First Military Medical University, Guangzhou 510515, China. yangliutcm@21cn.com
Objective:
To investigate the effects of Hominis placenta on melanocytes and tyrosinase activity in vitro.
Methods:
MTT assay was used to assess the proliferation of melanocytes treated with Hominis placenta. Hunt method was employed to determine melanin synthesis and the oxidation rate of DL-dopa to evaluate the tyrosinase activity.
Results:
Hominis placenta promoted melanocyte proliferation (P<0.01) and significantly increased the cell number (P<0.01), melanin synthesis (P<0.01), with the optimal concentration of 0.1g/L (P<0.01), and also enhanced tyrosinase activity (P<0.01) at the optimal concentration of 1 g/L (P<0.01).
Conclusion:
Hominis placenta promotes proliferation, melanin synthesis and tyrosinase activity of the melanocytes.

