Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epigenome changes in active and inactive polycomb-group-controlled regions.

Achim Breiling1, Laura P O'Neill, Donatella D'Eliseo

  • 1Dulbecco Telethon Institute, Institute of Genetics and Biophysics CNR, Via Pietro Castellino 111, 80131 Naples, Italy.

EMBO Reports
|September 28, 2004
PubMed
Summary

Polycomb repressive complex 1 (PRC1) maintains gene silencing. Active promoters have low PRC1 levels, while repressed regions show specific histone trimethylation marks, indicating their role in epigenetic inheritance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

T cells contribute to approaching positive but potentially risky stimuli through medial prefrontal cortex immune modulation.

Brain, behavior, and immunity·2026
Same author

PRC2-EZH1 contributes to circadian gene expression by orchestrating chromatin states and RNA polymerase II complex stability.

The EMBO journal·2024
Same author

Author Correction: LINE-1 RNA triggers matrix formation in bone cells via a PKR-mediated inflammatory response.

The EMBO journal·2024
Same author

LINE-1 RNA triggers matrix formation in bone cells via a PKR-mediated inflammatory response.

The EMBO journal·2024
Same author

TDP-43 Epigenetic Facets and Their Neurodegenerative Implications.

International journal of molecular sciences·2023
Same author

Synaptic and transcriptomic features of cortical and amygdala pyramidal neurons predict inefficient fear extinction.

Cell reports·2023

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genetics

Background:

  • Polycomb group (PcG) proteins mediate epigenetic inheritance of transcriptional repression.
  • In Drosophila, Polycomb repressive complex 1 (PRC1) silences genes by inhibiting transcription and chromatin remodeling at core promoters.

Purpose of the Study:

  • To map the distribution of PRC1 components, Enhancer of zeste (E(z)), and histone H3 modifications in active and inactive PcG-controlled regions.
  • To understand the role of E(z) histone methyl transferase (HMT) in establishing and maintaining PcG-mediated repression.

Main Methods:

  • Chromatin immunoprecipitation (ChIP) of in vivo formaldehyde-fixed chromatin.
  • Analysis of phenotypically diverse cultured Drosophila cell lines.
  • Mapping of PRC1 components, E(z) HMT, and histone H3 modifications (trimethylation at lysines 9 and 27).

Related Experiment Videos

Main Results:

  • PRC1 components are present in both active and inactive PcG-controlled regions, but at varying levels.
  • Active target promoters exhibit significantly lower levels of E(z) and Polycomb.
  • Inactive PcG-controlled regions are marked by trimethylation at H3 lysine 9 and lysine 27 (H3K9me3, H3K27me3).

Conclusions:

  • Histone trimethylation marks (H3K9me3, H3K27me3) are specific to inactive PcG-controlled regions.
  • The E(z) HMT is crucial for establishing and maintaining these PcG-specific repressive marks, thereby ensuring epigenetic inheritance of gene silencing.