Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mapping Ds insertions in barley using a sequence-based approach.

L D Cooper1, L Marquez-Cedillo, J Singh

  • 1Department of Crop and Soil Science, Oregon State University, OR 97331, Corvallis, USA.

Molecular Genetics and Genomics : MGG
|September 28, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inheritance and expression of NAD(P)H nitrate reductase in barley.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Cultivar-related differences in the distribution of cell-wall-bound thionins in compatible and incompatible interactions between barley and powdery mildew.

Planta·2013
Same author

Factors affecting anther culturability of recalcitrant barley genotypes.

Plant cell reports·2013
Same author

Improvement of anther culture methods for doubled haploid production in barley breeding.

Plant cell reports·2013
Same author

Localization of NAD(P)H-bispecific nitrate reductase genes to chromosomes of barley, rye, wheat and Aegilops umbellulata.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same journal

A CLN8 biallelic missense variant causes epilepsy with severe treatment-resistant psychosis.

Molecular genetics and genomics : MGG·2026
Same journal

Expression, prognostic value and function of LMBR1L in gastric cancer.

Molecular genetics and genomics : MGG·2026
Same journal

HIPK3 silencing promotes M2 macrophage polarization through NF-κB signaling pathway to enhance oral cancer progression.

Molecular genetics and genomics : MGG·2026
Same journal

Comparison of microbial profiles between hospital wastewater and river water.

Molecular genetics and genomics : MGG·2026
Same journal

Genetic landscape of the Brahmin population of Gujarat, India, using X-chromosome STR markers.

Molecular genetics and genomics : MGG·2026
Same journal

RUNX2 influences alternative splicing of genes associated with gastric cancer progression by regulating SF3B6 expression in HGC-27 cells.

Molecular genetics and genomics : MGG·2026
See all related articles

A new transposon tagging system using maize Ac/Ds elements was developed in barley. This system facilitates gene discovery and reverse genetics by creating Ds insertion lines throughout the barley genome.

Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Maize Ac/Ds elements offer a robust system for transposon tagging.
  • Developing such a system in barley is crucial for functional genomics in Triticeae.

Purpose of the Study:

  • To establish a transposon tagging system in barley using maize Ac/Ds elements.
  • To generate a collection of barley lines with Ds insertions dispersed across the genome for gene discovery.

Main Methods:

  • Biolistic transformation of barley with AcTPase and Ds-bar elements.
  • Inverse PCR and TAIL-PCR for flanking sequence identification.
  • Sequence-based mapping using SNP and indel polymorphisms in the Oregon Wolfe Barley population.

Main Results:

Related Experiment Videos

  • 19 independent barley lines with stabilized Ds insertions at new genomic locations were generated.
  • Genome locations of Ds insertions were determined using sequence-based mapping and confirmed with RFLP analysis.
  • BLAST searches revealed that most Ds insertions are within known or predicted coding sequences.

Conclusions:

  • The Ac/Ds transposon tagging system is effective in barley.
  • This system provides a valuable tool for functional genomics and gene discovery in barley and other Triticeae species.