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Related Experiment Videos

Optical manipulation in combination with multiphoton microscopy for single-cell studies.

Mattias Goksör1, Jonas Enger, Dag Hanstorp

  • 1Department of Experimental Physics, Chalmers University of Technology and Göteborg University, SE-412 96 Göteborg, Sweden. mattias.goksor@fy.chalmers.se

Applied Optics
|September 29, 2004
PubMed
Summary

Optical tweezers integrated with multiphoton microscopy enable 3D imaging of trapped yeast cells. This technique also utilizes the laser as scissors to create holes in sperm membranes.

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Area of Science:

  • Biophysics
  • Optical Physics
  • Cell Biology

Background:

  • Multiphoton microscopy offers deep tissue penetration and reduced phototoxicity for cellular imaging.
  • Optical tweezers provide precise manipulation of microscopic objects, including biological cells.

Purpose of the Study:

  • To integrate optical tweezers with multiphoton microscopy for 3D imaging of trapped cells.
  • To demonstrate a novel method for axial manipulation of trapped cells.
  • To explore the use of femtosecond lasers as both imaging and microsurgery tools.

Main Methods:

  • Utilized a continuous-wave (cw) 1064 nm Nd:YVO4 laser to generate optical tweezers for trapping live yeast cells.
  • Employed a pulsed femtosecond laser for 3D multiphoton imaging of the 4',6-diamidino-2-phenylindole-stained nucleus.

Related Experiment Videos

  • Controlled axial movement of trapped cells by adjusting an external lens to modify the trapping laser beam's divergence.

    • Main Results:

    • Successfully demonstrated 3D imaging of yeast cells held by optical tweezers.
    • Developed a simple method for integrating optical tweezers into laser scanning confocal and multiphoton microscopes.
    • Showcased the femtosecond laser's dual capability for imaging and laser microsurgery, creating holes in spermatozoa membranes.

    Conclusions:

    • The integration of optical tweezers with multiphoton microscopy provides a powerful platform for 3D cellular imaging and manipulation.
    • This combined technique offers a versatile tool for various biological applications, including cell imaging and membrane modification.
    • The study highlights the potential of femtosecond lasers in advanced microscopy and cellular surgery.