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Updated: Aug 21, 2026

An Experimental Human DIEP Flap Model to Investigate Preservation Strategies for Vascularized Composite Allografts and Free Flaps
Published on: December 5, 2025
[Hemodynamic study of an over-midline axial flap]
Ran Huo1, Sen-kai Li, Yang-qun Li
1Department of Plastic Surgery and Burns, Shandong Province Hospital, Jinan 250021, China.
Objective:
To investigate the hemodynamic changes of an over-midline axial flap.
Methods:
An over-midline axial flap, based on the iliac artery and veins, was raised on the back of each selected Wistar rat (n = 30), vascularied by contralateral. The flap size is 7 cm x 3 cm. The over-midline part is 3 cm x 3 cm. A laser doppler flowmetry (LDF) was used to evaluate the hemodynamic changes preoperatively, and 6 hours, 24 hours, 48 hours, 72 hours, 7 days, and 14 days after the operation respectively.
Results:
All of the flaps survived well. But, in the first three days after the operation, the blood perfusion of the flap in the areas of the midline and the distal part was significantly lower than the preoperation's level. Thereafter, the values of the blood perfusion started to increase till to the maximum 7 days after the operation. On the 14 days after the operation, the vascular structures was axially matched to the base of the flap. In the distal part of the flap, the choke vessels were gradually opened and resulted in the blood perfusion to the normal level.
Conclusion:
The hemodynamic changes of the over-midline axial flap transmidline flap could occur to match the need of the base of the flap.

