Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pure translation display.

Anthony C Forster1, Virginia W Cornish, Stephen C Blacklow

  • 1Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA. aforster@rics.bwh.harvard.edu

Analytical Biochemistry
|September 29, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CCL19<sup>+</sup> fibroblasts define a proliferative niche in chronic lymphocytic leukemia.

bioRxiv : the preprint server for biology·2026
Same author

High-throughput biochemical phenotyping of SHP2 variants reveals the molecular basis of diseases and allosteric drug inhibition.

bioRxiv : the preprint server for biology·2026
Same author

Adhesion G protein-coupled receptors.

Pharmacological reviews·2026
Same author

Activating Ras-MAPK pathway variants drive hippocampal clonal competition in human epilepsy.

bioRxiv : the preprint server for biology·2026
Same author

Design of soluble Notch agonists that drive T cell development and boost immunity.

Cell·2025
Same author

Creating and understanding new-to-nature chemistry.

Current opinion in chemical biology·2025
Same journal

Expression of concern: "Specific detection of Mycobacterium sp. genomic DNA using dual labeled gold nanoparticle based electrochemical biosensor" [Anal. Biochem. 417 (1) (2011) 73-79].

Analytical biochemistry·2026
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
See all related articles

Researchers developed a pure translation system to overcome limitations in creating unnatural amino acid polymers. This method enables the selection and directed evolution of novel peptide analogs for various applications.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Genetic selection methods like phage display are limited for unnatural amino acid polymers due to in vivo translation competition.
  • Existing methods struggle to incorporate unnatural amino acids into proteins during selection processes.

Purpose of the Study:

  • To develop a novel in vitro translation system for selecting polymers containing unnatural amino acids.
  • To enable the directed evolution of peptides with enhanced or novel properties using unnatural amino acids.

Main Methods:

  • Developed a pure translation system by omitting competing aminoacyl-tRNA synthetases and other cellular components.
  • Demonstrated "pure translation display" by selecting mRNA encoding specific unnatural amino acids linked to a peptide spacer.

Related Experiment Videos

Main Results:

  • Successfully synthesized long polypeptides using the pure translation system.
  • Showcased the ability to select specific mRNA molecules from a library based on the incorporation of unnatural amino acids.

Conclusions:

  • Pure translation display overcomes limitations of in vivo systems for unnatural amino acid incorporation.
  • This technology facilitates the directed evolution of peptide analogs with potential catalytic or pharmacological functions.