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Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
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Latex antigens.

J E Slater1, S K Chhabra

  • 1Center for Cancer and Transplantation Biology, Children's Research Institute, Washington, D.C.

The Journal of Allergy and Clinical Immunology
|March 1, 1992
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Summary
This summary is machine-generated.

Children with spina bifida often have allergies to natural rubber latex proteins. This study identified a 14 kDa peptide in latex as a major allergen, crucial for understanding rubber allergy in this population.

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Area of Science:

  • Immunology
  • Allergy Research
  • Biochemistry

Background:

  • Some children with spina bifida exhibit IgE antibodies to natural rubber latex proteins.
  • Understanding the specific latex antigens responsible for allergic reactions is crucial for diagnosis and management.

Purpose of the Study:

  • To compare different sources of latex antigen.
  • To identify specific antigenic peptides in natural rubber latex using radioimmunoblotting.

Main Methods:

  • Sera from 26 children with spina bifida were tested for IgE reactivity to ammoniated latex (AL) and nonammoniated latex (NAL) extracts.
  • Proteins were separated by SDS-PAGE, transferred to PVDF membranes, and analyzed by radioimmunoblotting with patient sera.
  • Radioallergosorbent assay (RAST) and immunoblotting techniques were employed to detect IgE binding.

Main Results:

  • 18 out of 26 patients with spina bifida were positive for IgE to AL.
  • All tested RAST-positive sera showed IgE binding to a 14 kDa peptide in both AL and NAL.
  • The IgE binding to the 14 kDa peptide was more pronounced with reduced NAL, suggesting its significance as a major antigen.

Conclusions:

  • A 14 kDa peptide in natural rubber latex is identified as a major antigen in latex allergy.
  • Ammoniated latex is a suitable antigen source for in vitro diagnostic studies of latex allergy.
  • These findings contribute to a better understanding of rubber allergy in children with spina bifida.