Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drugs Used in Lower Respiratory Disorders: Overview01:17

Drugs Used in Lower Respiratory Disorders: Overview

1.6K
Lower respiratory tract disorders present challenges that often require skilled and nuanced approaches for effective management. Common ailments, such as asthma and chronic obstructive pulmonary disease (COPD), have prompted the development of intricate treatment strategies involving bronchodilators and anti-inflammatory drugs, each tailored to ease breathing and revitalize the lungs.
Bronchodilators, the first step of respiration enhancement, come in various forms, each with its own mechanism...
1.6K
Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

1.5K
Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
1.5K
Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids01:25

Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids

2.1K
Inhaled corticosteroids (ICS) are anti-inflammatory drugs used primarily in treating persistent asthma and providing long-term maintenance. They target the bronchial mucosa, the lining of the airways, to control inflammation, a critical factor in asthma progression and exacerbation.
ICS work through a multifaceted mechanism of action. They suppress the inflammatory response caused by the proliferation of TH cells. They also reduce the transcription of the IL-2 gene, which is involved in the...
2.1K
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

2.4K
Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
2.4K
Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

4.7K
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
4.7K
Asthma I: Introduction01:28

Asthma I: Introduction

311
Asthma is a chronic inflammatory disorder of the airways characterized by variable airflow obstruction and heightened bronchial responsiveness to a wide range of triggers. The underlying inflammation leads to airway swelling, mucus hypersecretion, and smooth muscle constriction, all of which narrow the airway lumen and impede airflow. Clinically, asthma presents with recurrent episodes of wheezing, shortness of breath, chest tightness, and coughing, symptoms that typically vary in intensity and...
311

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Consensus guidelines for interventional cardiology services delivery during covid-19 pandemic in Australia and new Zealand.

Heart, lung & circulation·2020
Same author

Pediatric heart transplantation across a positive crossmatch: First year results from the CTOTC-04 multi-institutional study.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2018
Same author

Incidence, characterization, and impact of newly detected donor-specific anti-HLA antibody in the first year after pediatric heart transplantation: A report from the CTOTC-04 study.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2018
Same author

Lessons Learned: Early Termination of a Randomized Trial of Calcineurin Inhibitor and Corticosteroid Avoidance Using Belatacept.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
Same author

Altered composition of epidermal lipids correlates with Staphylococcus aureus colonization status in atopic dermatitis.

The British journal of dermatology·2017
Same author

Multicenter Analysis of Immune Biomarkers and Heart Transplant Outcomes: Results of the Clinical Trials in Organ Transplantation-05 Study.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015

Related Experiment Video

Updated: May 5, 2026

Murine Model of Allergen Induced Asthma
08:05

Murine Model of Allergen Induced Asthma

Published on: May 14, 2012

40.2K

Steroid-resistant asthma: immunologic and pharmacologic features.

J Alvarez1, W Surs, D Y Leung

  • 1Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.

The Journal of Allergy and Clinical Immunology
|March 1, 1992
PubMed
Summary

Patients with steroid-resistant asthma show increased T cell proliferation in response to glucocorticoids. This cellular abnormality may explain why some asthma patients do not respond well to steroid treatments.

More Related Videos

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

7.0K
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
10:25

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Published on: September 20, 2019

6.5K

Related Experiment Videos

Last Updated: May 5, 2026

Murine Model of Allergen Induced Asthma
08:05

Murine Model of Allergen Induced Asthma

Published on: May 14, 2012

40.2K
Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

7.0K
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
10:25

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Published on: September 20, 2019

6.5K

Area of Science:

  • Immunology
  • Pharmacology
  • Respiratory Medicine

Background:

  • Glucocorticoids are crucial for asthma management.
  • A subset of asthma patients exhibit poor responsiveness to glucocorticoid therapy, termed steroid-resistant (SR) asthma.

Purpose of the Study:

  • To investigate the immunologic and pharmacologic characteristics of patients with SR asthma.
  • To compare cellular responses in SR asthma patients to steroid-sensitive (SS) asthma patients and healthy controls.

Main Methods:

  • Evaluated 17 SR asthma patients, 24 SS asthma patients, and 47 healthy controls.
  • Assessed peripheral blood mononuclear cells (PBMCs) for DNA synthesis and T cell proliferation in response to methylprednisolone (Mpn) and phytohemagglutinin.
  • Steroid pharmacokinetics were assessed in a subset of SR asthma patients.

Main Results:

  • Patients with SR asthma showed significantly increased T cell proliferation in PBMCs when stimulated with Mpn compared to SS patients and controls.
  • Steroid pharmacokinetics were within normal limits in the evaluated SR asthma patients.
  • The augmented DNA synthesis in SR asthma PBMCs was reversible in vitro with troleandomycin.

Conclusions:

  • Peripheral blood mononuclear cells from SR asthma patients exhibit an altered response to methylprednisolone.
  • This cellular abnormality in T cell response may contribute to persistent airway inflammation in SR asthma despite glucocorticoid treatment.