Related Experiment Video
Updated: Aug 21, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
A nucleotide binding motif in hepatitis C virus (HCV) NS4B mediates HCV RNA replication
Shirit Einav1, Menashe Elazar, Tsafi Danieli
1Division of Gastroenterology and Hepatology, Stanford University School of Medicine, CCSR Building, Room 3115, 269 Campus Drive, Palo Alto, CA 94305-5187, USA.
Abstract:
Hepatitis C virus (HCV) is a major cause of viral hepatitis. There is no effective therapy for most patients. We have identified a nucleotide binding motif (NBM) in one of the virus's nonstructural proteins, NS4B. This structural motif binds and hydrolyzes GTP and is conserved across HCV isolates. Genetically disrupting the NBM impairs GTP binding and hydrolysis and dramatically inhibits HCV RNA replication. These results have exciting implications for the HCV life cycle and novel antiviral strategies.
More Related Videos
Related Concept Videos
Viruses with RNA Genomes
Hepatitis
Inhibitors Of Virion Release
Antiviral Nucleoside Inhibitors
Retrovirus Life Cycles
Inhibitors of Viral Protein Synthesis

