Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selection and evolution of NTP-specific aptamers.

Laure Weill1, Dominique Louis, Bruno Sargueil

  • 1Centre de Génétique Moléculaire, CNRS, Avenue de la Terrasse, 91190 Gif sur Yvette, France.

Nucleic Acids Research
|September 29, 2004
PubMed
Summary

Researchers developed new RNA aptamers that bind Adenosine Triphosphate (ATP) by recognizing its sugar-phosphate backbone. This advances understanding of the RNA world hypothesis and enzyme development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combining SMN2 splicing modifiers with HDAC6 inhibition improves spinal muscular atrophy outcomes.

Brain : a journal of neurology·2026
Same author

Designing molecular RNA switches with Restricted Boltzmann machines.

Nature communications·2025
Same author

Drug Candidate BIO101 for Spinal Muscular Atrophy as Monotherapy or Combined With the Antisense Oligonucleotide ASO-10-27.

Journal of cachexia, sarcopenia and muscle·2025
Same author

DEAD-box helicase intrinsically disordered domains and structural dynamics of HIV-1 RNA are required to reveal DDX3X catalytic efficiency.

Nucleic acids research·2025
Same author

A Multiscale Study to Characterize SHAPE Probe/RNA Molecules Prereactive Complex and Its Role in Initiating the SHAPE Reaction.

Journal of chemical information and modeling·2025
Same author

Investigation of a squaramide motif as a bioisostere of the amino-acid group of S-adenosyl-L-methionine and its functional impact on RNA methylation.

Communications chemistry·2025

Area of Science:

  • Biochemistry
  • Molecular Biology
  • RNA Therapeutics

Background:

  • Adenosine Triphosphate (ATP) is crucial in biology as an RNA building block and universal cofactor.
  • In vitro molecular evolution frequently targets ATP-binding motifs.
  • Understanding ATP binding is key to the RNA world hypothesis and novel enzyme design.

Purpose of the Study:

  • To isolate novel RNA aptamers binding ATP in distinct orientations.
  • To explore new ATP-binding motifs beyond known adenosine or Hoogsteen face recognition.
  • To investigate aptamer specificity for ATP derivatives and implications for molecular evolution.

Main Methods:

  • Selection of RNA aptamers using affinity chromatography with ATP immobilized in three orientations.
  • Characterization of isolated ATP-binding RNA motifs.
  • Secondary selection for cordycepin specificity.

Main Results:

  • Five new RNA motifs binding ATP were isolated.
  • All selected aptamers recognize the sugar-phosphate backbone of nucleotides.
  • Three aptamers showed selectivity for adenine derivatives; others recognized all four nucleotides.

Conclusions:

  • Novel ATP-binding RNA aptamers targeting the sugar-phosphate backbone were discovered.
  • These findings contribute to the study of the RNA world and the development of new enzymes.
  • The characterized aptamers offer insights into molecular evolution strategies.

Related Experiment Videos