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[Anticoagulants preventing pseudo-thrombocytopenia]
1Department of Clinical Laboratory and Medicine, Kyoto Prefectural University of Medicine.
Rinsho Byori. the Japanese Journal of Clinical Pathology
|January 1, 1992
Summary
Ethylenediamine tetraacetic acid (EDTA) can cause false low platelet counts. A new method combining EDTA with citric acid effectively prevents this pseudo-thrombocytopenia without altering other blood cell parameters.
Area of Science:
- Hematology
- Clinical Pathology
Context:
- Pseudo-thrombocytopenia, often caused by ethylenediamine tetraacetic acid (EDTA), leads to inaccurate platelet counts.
- Accurate platelet enumeration is critical for diagnosing and managing various hematological conditions.
Purpose:
- To evaluate anticoagulants that prevent pseudo-thrombocytopenia while preserving other hematological data.
- To assess the efficacy of a novel anticoagulant combination (EDTA and citric acid) for platelet count stability.
Summary:
- A new method using EDTA-2K (10 mg/ml) and citric acid (1 mg/ml) of blood was tested.
- This EC method successfully inhibited thrombocyte aggregation and maintained stable platelet counts in patients with pseudo-thrombocytopenia.
- The EC method did not impact other blood cell counts, cell morphology, or stainability, and preserved micro-hematocrit values.
Impact:
- This EDTA-citric acid combination offers a reliable solution for preventing pseudo-thrombocytopenia in laboratory diagnostics.
- It ensures accurate hematological data, improving diagnostic reliability and patient care.
- The method demonstrates stability of hematological parameters for at least 24 hours post-sampling.