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Updated: Aug 10, 2026

RhoC GTPase Activation Assay
Published on: August 23, 2010
Activation of gef-h1, a guanine nucleotide exchange factor for RhoA, by DNA transfection
Marcus Brecht1, Agatha C M Steenvoorden, John G Collard
1University of Heidelberg, Institute of Human Genetics, Im Neunheimer Feld 366, D-69120 Heidelberg, Germany.
Abstract:
Several oncogenes isolated by the NIH/3T3 transformation assay, i.e., dbl, dbs, lbc, lfc, lsc, net, ost and tim, contain a Dbl homology (DH) and a pleckstrin-homology (PH) domain and act as GEFs (guanine nucleotide exchange factors) for Rho-like GTPases. In a search for genes with oncogenic potential in DNA from the monocytic leukaemia cell line U937, we identified an amino-terminal truncated form of gef-h1, a gene encoding a GEF for RhoA. These data support the idea that a systematic search for mutations and/or deletions of GEFs in human cancer is promising.
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