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[Determination of tetanus toxin and toxoid by ELISA using monoclonal antibodies]

M A Burkin, V V Sviridov, O V Perelygina

    Prikladnaia Biokhimiia I Mikrobiologiia
    |October 1, 2004
    PubMed

    The procedure of obtaining monoclonal antibodies TT-1, TT-2, and TT-3 against tetanus toxin/toxoid is described. It is shown that both commercial DTP vaccine and tetanus toxoid conjugated with a low-molecular-weight hapten can be used an immunogens. Monoclonal antibodies TT-1 and TT-2 neutralized tetanus toxin in vivo. The monoclonal antibodies obtained were used to design and compare several schemes of quantitative determination of tetanus toxoid and toxin by ELISA. A more sensitive competitive ELISA allowed detecting as much as 0.01 EC/ml toxoid and 50 LD50/ml toxin.

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