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Updated: Aug 13, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
Published on: July 17, 2012
Absorption and scattering imaging of tissue with steady-state second-differential spectral-analysis tomography
Heng Xu1, Brian W Pogue, Hamid Dehghani
1Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA. heng.xu@dartmouth.edu
Abstract:
A novel approach to reconstructing both the absorption and the scattering properties of a turbid medium simultaneously from steady-state broadband spectral measurements is presented that utilizes second-differential fitting to the water spectrum to estimate the optical path length in tissue. Theoretical and experimental evidence is provided to demonstrate the robust accuracy of the spectroscopy approach and reconstructed absorption images. The steady-state broadband CCD system has the potential to provide accurate chromophore imaging without the technological complexity of time- or frequency-domain systems.
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