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Monoallelic expression of mouse Cd4 gene
Rosanna Capparelli1, Adele Costabile, Maurizio Viscardi
1School of Biotechnological Sciences, University of Naples Federico II, Italy.
Summary
Single-cell analysis reveals that CD4+ lymphocytes express only one parent
Area of Science:
- Immunology
- Genetics
- Molecular Biology
Background:
- The CD4 gene is crucial for T-cell function.
- Understanding allele expression in hybrid mice provides insights into gene regulation.
Purpose of the Study:
- To investigate the parental origin of expressed CD4 alleles in single lymphocytes.
- To determine if CD4 gene expression is monoallelic in F1 hybrid mice.
Main Methods:
- Utilized a 7-bp deletion in the Cd4 gene of MOLF/Ei mice for allele discrimination.
- Employed single-cell RT-PCR to analyze Cd4 expression in individual lymphocytes.
- Used in situ hybridization to examine Cd4 allele replication patterns.
Main Results:
- Single CD4+ lymphocytes exclusively express either the maternal or paternal Cd4 allele, not both.
- Cd4 alleles demonstrated asynchronous replication, consistent with monoallelic expression.
Conclusions:
- CD4 gene expression in individual lymphocytes is strictly monoallelic.
- This monoallelic expression is linked to asynchronous replication of Cd4 alleles.