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Related Experiment Videos

[Construction and expression of basic fibroblast growth factor mammalian expression vector].

Qixu Zhang1, Gang Zhou, Jiayun Lu

  • 1Scar Multiple Treatment Center, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100041, PR China.

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery
|October 6, 2004
PubMed
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A new mammalian expression vector for basic fibroblast growth factor (bFGF) was successfully constructed and demonstrated effective in vitro and in vivo expression, supporting tissue engineering applications.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Gene Therapy

Background:

  • Basic fibroblast growth factor (bFGF) is crucial for cell growth and tissue repair.
  • Developing effective gene delivery systems for bFGF is essential for therapeutic applications.

Purpose of the Study:

  • To construct a mammalian expression vector for bFGF.
  • To evaluate the in vitro and in vivo expression of bFGF.
  • To assess the potential of bFGF gene therapy in tissue engineering.

Main Methods:

  • Gene cloning was used to construct the pcDNA3.1/myc-His(-)C-bFGF vector.
  • Expression was verified using RT-PCR and immunohistochemistry in vitro.
  • Recombinant plasmids were delivered to rabbit muscle via electroporation for in vivo analysis.

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Main Results:

  • The constructed vector successfully expressed bFGF in cultured cells.
  • High-level expression of bFGF and VEGF121 was achieved in vivo.
  • Significant improvements in blood vessel formation and circulation were observed.

Conclusions:

  • The developed bFGF expression vector is effective for both in vitro and in vivo applications.
  • This vector serves as a foundational tool for bFGF-based gene therapy in tissue transplantation and engineering.