Related Experiment Video
Updated: Aug 21, 2026

Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice
Published on: January 16, 2015
[Expression of MCP-1 in airway of rats with COPD]
Hui Wang1, De-yun Cheng, Jian Guan
1Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To explore the role of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of chronic obstructive plumonary disease (COPD).
Methods:
Rat COPD model was established by exposing the rats to cigarette smoke daily. After COPD model was set up, bronchoalveolar lavage was performed. Total cell counts and alveolar macrophage (AM) counts in bronchoalveolar lavage fluid (BALF) were examined and the levels of MCP-1 and tumor necrosis factor-alpha (TNF-alpha) in BALF and serum were measured by ELISA. Lung tissue sections stained by HE were measured in terms of MLI, MAN and PAA by picture analysis system.
Results:
MLI and PAA in COPD model were higher than those of control group while MAN was just on the contrary. Except MCP-1 levels in serum [(32.75 +/- 10.91) pg/ml vs (24.13 +/- 6.92) pg/ml, P>0.05], MCP-1 levels, TNF-alpha levels, total cell counts and AM counts in BALF of COPD model group were significantly higher than those of control group (P<0.01). Positive correlations were demonstrated among MCP-1 levels, TNF-alpha levels and AM counts in BALF in COPD group, but were not demonstrated between MCP-1 levels, TNF-alpha levels in serum and AM counts.
Conclusion:
There is a close correlation between MCP-1 in BALF and airway inflammation in COPD.
Related Concept Videos
Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features
Chronic Obstructive Pulmonary Disease II: Emphysema
Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies
Medical History
