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Published on: August 14, 2018
[TK locus mutation assay: comparison of L5178Y and TK6 cell lines]
Pei-qiang Shuai1, Li-shi Zhang, Yi-jing Wang
1Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan, University, Chengdu 610041, China.
Objective:
To compare the advantages and disadvantages of utilizing L5178Y and TK6 cell lines in the TK locus mutation assay.
Methods:
The two cell lines were used for detecting and assessing the mutability of four chemicals (MMS, EMS, MMC and KCl); the microwell method of TK locus mutation assay was adopted.
Results:
The two cell lines brought about similar results in the study. The tolerance of TK6 to all four chemicals was lower than that of L5178Y, and all the relative mutation indices of TK6 were higher than those of L5178Y.
Conclusion:
Each of the two cell lines has its own strong points; nevertheless, the authors recommend the applications of TK6 cell in the assay system since this cell line comes from human.
