[Construction of the subtracted cDNA library in hypothalamus of the seasickness adaptive rats]
Related Experiment Video
Updated: Aug 21, 2026
Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD
Published on: October 10, 2012
Yanhong Ji1, Junsheng Guo, Min Li
1Department of Military Hygiene, the Second Military Medical University, Shanghai 200433, China.
Objective:
To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness.
Methods:
Suppression subtract hybridization technique was used, and forward and reverse hybridization was performed on the hypothalamus of seasickness adaptive rats and that of normal rats so that to construct subtracted cDNA library. Dot blot was used for differential screening the subtracted library.
Results:
23 fragments of differentially expressed genes was obtained including 10 up-regulating and 13 down-regulating fragments.
Conclusion:
Many played role in adaptability formation to seasickness such as SAM, vasopressin, and heme oxygenase.
