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Updated: Aug 21, 2026
An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Published on: October 4, 2018
Molecular characterization of an Infectious bursal disease virus isolate from Iran
S D Hosseini1, A R Omar, I Aini
1Research Center of Animal Science and Natural Resources, Arak, Iran.
Abstract:
The segment A of an Infectious bursal disease virus (IBDV) isolate from Iran was amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced and compared with published sequences of 26 IBDV isolates from other parts of the world. The Iranian isolate showed 8 unique amino acid differences. In addition, 9 common amino acid differences, namely 3 in VP2, (222 Ala, 256 lIe and 294 lIe), 3 in VP4 (685 Asn/Ser, 715 Ser and 751 Asp), 2 in VP3 (990 Val and 1005 Ala), and 1 in VP5 (49 Arg) were found. Phylogenetic analysis indicated that the Iranian isolate is closely related to highly virulent (hv) IBDV isolates from Asian countries. Nevertheless, it may share a common origin with hv isolates from other parts of the world.
