Transformation of Caenorhabditis elegans with genes from parasitic nematodes

Our knowledge of many aspects of the molecular biology of animal parasitic nematodes has rapidly expanded in recent years but the classical genetic analysis of this group of organisms has yet to emerge as a viable discipline. For example, it is not possible to routinely perform crosses between single males and females to examine the genetic basis of even simple phenotypes such as anthelmintic resistance. This has meant that the function of many cloned parasite genes can only be inferred from sequence comparison with genes from other organisms where the function is known, or by correlation of DNA polymorphisms linked to the gene with phenotypic differences between strains or individuals. In the absence of classical genetic techniques, a molecular solution is to transform a suitable host with the gene of interest, but what defines a suitable host? Here, Warwick Grant describes recent work that aims to provide such a host.