Antibody formation to dog pulp tissue altered by Grossman's Formula Sealer via the root canal

Pulp tissue from experimental dogs was tested for its antigenicity before and after incubation with Grossman's Formula Sealer (GS). Reactions to Arthus skin tests showed an increased response to pulp that had been incubated in GS, washed, and then injected, as compared with the material alone. Hemagglutinating antibody titers (1:160 to 1:360) were obtained from pulp tissue altered by GS. Inhibition tests showed the specificity of the antibody to be at a dilution titer of 1:200. Therefore, GS altered dog pulp tissue and rendered it antigenically active, and a specific humoral response was produced.