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Updated: Aug 21, 2026

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
09:02

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Published on: September 22, 2023

Airway epithelial cell-pathogen interactions

Alessandra Bragonzi1, Elena Copreni, Sophie de Bentzmann

  • 1Institut fur Allgemeine Hygiene und Umwelthygiene, Universität Tübingen, Tübingen, Germany.

Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society
|October 7, 2004
PubMed

Insights

Cystic fibrosis (CF) airways are prone to specific bacterial infections. This study details in vitro and in vivo models for studying Pseudomonas aeruginosa and Staphylococcus aureus interactions in CF lung environments.

Area of Science:

  • Microbiology
  • Pulmonology
  • Pathogen Research

Background:

  • Cystic fibrosis (CF) airways exhibit limited pathogen colonization.
  • Understanding bacterial-host interactions in CF is crucial for treatment development.

Purpose of the Study:

  • To describe in vitro methods for studying Pseudomonas aeruginosa (Pa) and Staphylococcus aureus (Sa) adherence and uptake by airway epithelial cells.
  • To consider acute and chronic respiratory infection models in CF transgenic mice that mimic human CF lung pathology.

Main Methods:

  • In vitro assays for bacterial adherence and uptake by airway epithelial cells.
  • Establishment and utilization of CF transgenic mouse models for respiratory infection studies.

Main Results:

  • Detailed methodologies for in vitro bacterial-host interaction studies are provided.
  • CF mouse models that replicate human CF lung pathology are discussed.

Conclusions:

  • The described models are essential for investigating bacterial pathogenesis in cystic fibrosis.
  • These models facilitate the study of Pseudomonas aeruginosa and Staphylococcus aureus in CF airways.

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