Related Experiment Video
Updated: Aug 21, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Alternative bioseparation operations: life beyond packed-bed chromatography
Todd M Przybycien1, Narahari S Pujar, Landon M Steele
1Carnegie Mellon University, Department of Biomedical Engineering, 5000 Forbes Avenue, Pittsburgh, PA 15213, USA.
Abstract:
Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of being the single largest cost center in downstream processing and of being a low-throughput operation. Consequently, 'chromatography alternatives' are an attractive proposition, even if only a reduction in the extent of use of packed beds can be realized. This paper reviews the current state of unit operations posing as chromatography alternatives--including membrane filtration, aqueous two-phase extraction, three-phase partitioning, precipitation, crystallization, monoliths and membrane chromatography--and their potential to do the unthinkable.
Related Concept Videos
Ion-Exchange Chromatography
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Affinity Chromatography
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Overview Of Cell Separation And Isolation
