Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Entropy production mapping on stretched DNA interacted with proteins.

Yoshihiro Takeda1, Fumitaka Mafuné, Tamotsu Kondow

  • 1East Tokyo Laboratory, Genesis Research Institute Inc., 717-86 Futamata, Ichikawa, Chiba 272-0001, Japan. takeda@clusterlab.jp

Journal of Biotechnology
|October 7, 2004
PubMed
Summary

This study introduces an entropy production mapping (EPM) method to detect DNA structural changes. The EPM method successfully identified histone interaction sites on lambda DNA by measuring increased orientational entropy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estimating nasal nitric oxide from measured fractional exhaled nitric oxide and nasal fractional exhaled nitric oxide.

Journal of thoracic disease·2026
Same author

Elicitation of allergic airway inflammation modulates the cough response via increased prostaglandin I₂ in ovalbumin-sensitized guinea pigs.

Prostaglandins & other lipid mediators·2026
Same author

Laboratory-based cellular-level correlative visible-light and X-ray microscopy for 3D evaluation of mouse kidney biopsy.

Scientific reports·2026
Same author

High-Contrast X-Ray Computed Tomography for Quantifying Amorphous Content in Melt-Quenched Acetaminophen.

AAPS PharmSciTech·2026
Same author

Size-Dependent Hydrogen Adsorption and Desorption by Gas-Phase Iron Cluster Cations Revealed by Thermal Desorption Spectrometry.

The journal of physical chemistry. A·2025
Same author

Observation of NO molecules adsorbed on Rh cluster anions at different temperatures using photoelectron spectroscopy.

Nanoscale·2025

Area of Science:

  • Biophysics
  • Molecular Biology
  • Genetics

Background:

  • DNA structure and function are critical in biological processes.
  • Understanding higher-order structural changes in DNA is essential for studying molecular interactions.
  • Existing methods may lack precision in identifying localized DNA structural alterations.

Purpose of the Study:

  • To develop and validate a novel method, entropy production mapping (EPM), for detecting higher-order structural changes in DNA.
  • To quantify changes in orientational entropy of Watson-Crick base pairs upon interaction with biological factors.
  • To demonstrate the EPM method's utility in distinguishing interaction sites on DNA.

Main Methods:

  • Developed an entropy production mapping (EPM) method to measure orientational entropy.

Related Experiment Videos

  • Applied EPM to stretched and immobilized DNA molecules on a cover slip.
  • Utilized a histone-lambda DNA system to test the EPM method's efficacy.

    • Main Results:

    • The EPM method successfully detected higher-order structure changes in DNA.
    • Increased orientational entropy was observed in regions of biological factor interaction.
    • Histone interaction sites on lambda DNA were clearly distinguished from non-interaction regions.

    Conclusions:

    • Entropy production mapping (EPM) is an effective technique for identifying localized structural changes in DNA.
    • The method provides a sensitive measure of DNA structural alterations induced by molecular interactions.
    • EPM offers a valuable tool for investigating DNA-protein interactions and their structural consequences.