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Updated: Jul 31, 2026

Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
Human myeloperoxidase catalyzes an oscillating peroxidase-oxidase reaction
Jens Christian Brasen1, Anita Lunding, Lars Folke Olsen
1CelCom, Institute of Biochemistry and Molecular Biology, Syddansk Universitet, Campusvej 55, DK-5230 Odense M, Denmark.
Abstract:
We have studied the peroxidase-oxidase reaction catalyzed by human myeloperoxidase in an open system where both substrates-molecular oxygen and NADH-are supplied continuously to the reaction mixture. The reaction shows oscillatory kinetics at pH values around 5, provided that the reaction medium in addition to the enzyme and the substrates also contains an aromatic electron mediator such as melatonin or 4-hydroxybenzoic acid and chloride ions at concentrations >1mM. The experimental findings can be simulated by a detailed model of the reaction. The results are important for our understanding of oxidant production in neutrophils.
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