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Related Experiment Videos

Rodent lymphocytes express functionally active glutamate receptors.

Alexander A Boldyrev1, Vasily I Kazey, Toomas A Leinsoo

  • 1Department of Biochemistry, Center for Molecular Medicine and International Biotechnological Center, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia.

Biochemical and Biophysical Research Communications
|October 7, 2004
PubMed
Summary
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Glutamate receptors in rodent lymphocytes regulate cell death and function. Activation of these receptors influences calcium, reactive oxygen species, and caspase-3, impacting lymphocyte longevity.

Area of Science:

  • Immunology
  • Neuroscience
  • Cell Biology

Background:

  • Glutamate receptors, typically studied in neurons, are increasingly recognized for roles beyond the central nervous system.
  • The presence and function of ionotropic (iGluR NR1) and metabotropic (mGluR Group III) glutamate receptors in rodent lymphocytes remain largely unexplored.

Purpose of the Study:

  • To investigate the expression and functional roles of iGluR NR1 and mGluR Group III glutamate receptors in rodent lymphocytes.
  • To elucidate the signaling pathways and cellular consequences of glutamate receptor activation in lymphocytes.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) for gene expression analysis.
  • Flow cytometry to assess intracellular calcium, reactive oxygen species (ROS), and caspase-3 activation.

Related Experiment Videos

  • Pharmacological manipulation using agonists (NMDA, L-AP4) and antagonists (MK-801), as well as N-acetylcysteine and cyclosporin A.

    • Main Results:

    • RT-PCR confirmed the expression of iGluR NR1 and mGluR Group III receptors in rodent lymphocytes.
    • NMDA activation of iGluR NR1 increased intracellular calcium and ROS, and triggered caspase-3 activation, which was inhibited by MK-801, N-acetylcysteine, and cyclosporin A.
    • L-AP4 activation of mGluR Group III increased ROS, and combined NMDA/L-AP4 treatment synergistically elevated ROS, leading to necrotic cell death without caspase-3 activation.

    Conclusions:

    • Rodent lymphocyte iGluR NR1 and mGluR Group III receptors are functional and play roles in regulating lymphocyte functions.
    • These receptors influence key cellular events including proliferation, maturation, and cell death pathways (apoptosis and necrosis).
    • Glutamate receptor signaling in lymphocytes may represent a novel target for modulating immune responses and cell longevity.