Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression of Stra13 during mouse endochondral bone development.

Helen E MacLean1, Henry M Kronenberg

  • 1Endocrine Unit, WEL501, Massachusetts General Hospital, 50 Blossom St, Boston, MA 02114, USA.

Gene Expression Patterns : GEP
|October 7, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intermittent parathyroid hormone employs autonomous and non-autonomous mechanisms to drive osteogenesis from Ebf3-expressing skeletal progenitor cells.

bioRxiv : the preprint server for biology·2026
Same author

Inducible activation of PKA in osteoblasts causes a profound high bone turnover phenotype similar to human diseases.

bioRxiv : the preprint server for biology·2026
Same author

A mouse model of Jansen's metaphyseal chondrodysplasia for investigating disease mechanisms and candidate therapeutics.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Single cell RNA sequencing shows that cells expressing Sox9 postnatally populate most skeletal lineages in mouse bone.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2025
Same author

Adult Prg4+ progenitors repair long-term articular cartilage wounds in vivo.

JCI insight·2023
Same author

Genome-wide CRISPR screening of chondrocyte maturation newly implicates genes in skeletal growth and height-associated GWAS loci.

Cell genomics·2023
Same journal

Distinct cellular and subcellular distributions of the alpha and beta isoforms of Heat Shock Protein 90 in the mouse cerebral cortex.

Gene expression patterns : GEP·2026
Same journal

Molecular characterization and expression dynamics of Labeo rohita MDA5, its adaptor MAVS and pro-inflammatory cytokines against PAMPs stimulation and pathogen challenge.

Gene expression patterns : GEP·2026
Same journal

Defining the spatiotemporal expression pattern of Rap1 in the chicken embryonic spinal cord.

Gene expression patterns : GEP·2026
Same journal

Expression analysis of the TMC gene family in the auditory system during zebrafish embryonic development.

Gene expression patterns : GEP·2026
Same journal

Mapping the locations of barx1-expressing prechondrogenic condensation domains in the posterior arches during ceratobranchial cartilage formation in zebrafish.

Gene expression patterns : GEP·2026
Same journal

cDNA cloning, expression analysis, and subcellular localization of TRIM13 gene in zebrafish.

Gene expression patterns : GEP·2026
See all related articles

The basic helix-loop-helix factor Stra13 is expressed in mouse bone development, particularly in hypertrophic chondrocytes. Its expression pattern overlaps with VEGF, a hypoxia-induced gene, suggesting a role in endochondral ossification.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Skeletal Biology

Background:

  • Endochondral ossification is a complex process involving precise regulation of chondrocyte differentiation.
  • The basic helix-loop-helix (bHLH) transcription factor Stra13 (also known as DEC1/Sharp2) plays roles in cell differentiation and proliferation.
  • Hypoxia is a known regulator of chondrocyte function during skeletal development.

Purpose of the Study:

  • To investigate the expression pattern of Stra13 during mouse endochondral bone development.
  • To compare Stra13 expression with that of VEGF, a hypoxia-inducible gene, in the developing growth plate.

Main Methods:

  • In situ hybridization was used to examine Stra13 mRNA expression in mouse tibia.
  • Expression analysis was performed at various embryonic (E14.5-E18.5) and post-natal (day 24) stages.

Related Experiment Videos

  • Co-localization studies with collagen X and VEGF expression were conducted.

    • Main Results:

    • Stra13 mRNA was detected in scattered hypertrophic chondrocytes at E14.5, increasing by E15.5 in post-mitotic hypertrophic chondrocytes.
    • From E16.5-E18.5, Stra13 was found in both proliferating and late hypertrophic chondrocytes, and in the primary spongiosa.
    • Stra13 expression persisted in late hypertrophic chondrocytes at post-natal day 24.
    • Stra13 and VEGF expression patterns showed significant overlap in chondrocytes during embryonic development.

    Conclusions:

    • Stra13 is dynamically expressed throughout endochondral bone development in mice.
    • The overlapping expression of Stra13 and VEGF suggests a potential role for Stra13 in mediating hypoxic responses within the growth plate.
    • These findings contribute to understanding the molecular mechanisms regulating chondrocyte differentiation and skeletal growth.