Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A model membrane protein for binding volatile anesthetics.

Shixin Ye1, Joseph Strzalka, Inna Y Churbanova

  • 1Department of Chemistry, Department of Anesthesiology, University of Pennsylvania, Philadelphia, Pennsylvania.

Biophysical Journal
|October 7, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-Organized Nanoplasmonic Artificial Leaf for Hot-Carrier Bioelectronic Interfaces.

Nature photonics·2026
Same author

Solid-State Side-Chain Functionalization of Conjugated Polymers for Expanded Chemical and Functional Versatility.

Journal of the American Chemical Society·2026
Same author

Exploring the Mechanism of Qigesan in Treating Esophageal Carcinoma Based on Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation.

Current computer-aided drug design·2026
Same author

Adsorption of Mixed Micelles of Polysorbate 80 and Oleic Acid to the Air-Water Interface.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Zwitterionic hydrogel designs for conducting polymers enable bioelectronics with suppressed foreign body responses.

bioRxiv : the preprint server for biology·2025
Same author

Screening and experimental validation of modified Gandou Decoction-targeted inhibitors for alleviating AD components via network pharmacology, machine learning, and molecular dynamics simulation.

Frontiers in pharmacology·2025

Researchers designed a new amphiphilic protein to model anesthetic binding in cell membranes. This protein, while showing altered halothane affinity, serves as a template for studying anesthetic-peptide interactions.

Area of Science:

  • Biophysics
  • Protein Design
  • Membrane Protein Mimicry

Background:

  • A water-soluble protein with a core cavity binds volatile anesthetics like halothane.
  • Understanding anesthetic binding requires a more relevant membrane protein model.

Purpose of the Study:

  • To engineer an amphiphilic four-helix bundle protein that mimics membrane proteins.
  • To investigate the impact of a hydrophobic domain on anesthetic binding affinity.
  • To establish a model system for studying anesthetic-peptide interactions.

Main Methods:

  • Synthesis of an amphiphilic four-helix bundle protein.
  • Formation of stable monolayers at the air/water interface.
  • Measurement of halothane binding affinity (Kd).

Related Experiment Videos

  • Specular X-ray reflectivity to determine protein orientation and length.

    • Main Results:

    • The amphiphilic protein forms stable monolayers and orients perpendicularly at the air/water interface.
    • Halothane binding affinity decreased to 3.1 mM Kd in the amphiphilic protein.
    • The presence of the core cavity is crucial for significant halothane binding.

    Conclusions:

    • The designed amphiphilic protein serves as a functional model for membrane-bound receptors.
    • This system provides a template for probing the physicochemical basis of anesthetic-peptide interactions.
    • Further studies can correlate structure and function to elucidate anesthetic binding mechanisms.