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Updated: Aug 21, 2026
Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker
Published on: September 15, 2023
Gene expression analysis in human gastric cancer cell line treated with trichostatin A and S-adenosyl-L-homocysteine
Heun-Sik Lee1, Mie-Hee Park, Suk-Jin Yang
1Laboratory of Human Genetics, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejon.
Abstract:
Trichostatin A (TSA) and S-adenosyl-L-homocysteine (AdoHcy) have been reported to affect histone modifications. To investigate the effects of two drugs that can reportedly affect chromatin remodeling, we analyzed the gene expression profiles of TSA and AdoHcy in a gastric cancer cell line using 14 K cDNA microarray. The significant analysis of microarray (SAM) identified 98 and 43 differentially expressed genes in TSA and AdoHcy treated sets, respectively, and selected genes were functionally classified. In the gastric cancer cell line, genes related to cell communication, cell growth/maintenance, and morphogenesis were highly expressed with TSA, and genes with cell growth/maintenance, metabolism, oxidoreductase activity were upregulated with AdoHcy. Genes downregulated with TSA included those controlling the cell cycle, cell growth/proliferation, DNA binding, and metabolism, whereas genes involved in calcium signaling, cell growth/proliferation, and metabolism were downregulated with AdoHcy. Furthermore, we identified the genes commonly expressed in both drug treatments. Compared to TSA, AdoHcy did not induce apoptosis in the SNU-16 gastric cancer cell line, and RT-PCR was performed for selective genes to confirm the microarray data. This gene expression profile analysis with TSA and AdoHcy should contribute to a greater understanding of the molecular mechanism of chromatin remodeling and cancer, and provide candidate genes for further studies involving the roles of histone modifications in gastric cancer.
Insights
Trichostatin A (TSA) and S-adenosyl-L-homocysteine (AdoHcy) alter gene expression in gastric cancer cells, impacting cell growth and metabolism. This study reveals key molecular mechanisms of chromatin remodeling in cancer.
Area of Science:
- Molecular Biology
- Cancer Research
- Epigenetics
Background:
- Histone modifications play a crucial role in chromatin remodeling.
- Trichostatin A (TSA) and S-adenosyl-L-homocysteine (AdoHcy) are known to influence histone modifications.
- Understanding their effects is vital for cancer research.
Purpose of the Study:
- To investigate the gene expression profiles of TSA and AdoHcy in a gastric cancer cell line.
- To identify differentially expressed genes and their functional classifications.
- To elucidate the molecular mechanisms of chromatin remodeling in gastric cancer.
Main Methods:
- Utilized 14K cDNA microarray to analyze gene expression profiles.
- Applied Significant Analysis of Microarray (SAM) to identify differentially expressed genes.
- Employed Reverse Transcription Polymerase Chain Reaction (RT-PCR) for data validation.
Main Results:
- TSA treatment upregulated genes related to cell communication, growth, and morphogenesis.
- AdoHcy treatment upregulated genes involved in cell growth, metabolism, and oxidoreductase activity.
- Both treatments affected cell cycle, DNA binding, and metabolic genes, with distinct downregulation patterns.
Conclusions:
- Gene expression profiling with TSA and AdoHcy provides insights into chromatin remodeling in gastric cancer.
- Identified candidate genes for further research on histone modifications in gastric cancer.
- AdoHcy did not induce apoptosis in the SNU-16 gastric cancer cell line.
